rabbit anti smad7  (Novus Biologicals)

Journal: Frontiers in Immunology

Article Title: GDF15 orchestrates mitochondrial-immune crosstalk via SMAD7-HIF-1α-PKM2 cascade to attenuate septic liver injury

doi: 10.3389/fimmu.2025.1712741

Figure Lengend Snippet: GDF15 preserves mitochondrial homeostasis in LPS-stimulated macrophages through dual regulation of SMAD7 and PKM2 pathways. (A) HIF-1α and SMAD7 expression in RAW264.7 macrophages across conditions: Untreated, LPS, LPS with rAAV8-mGdf15 overexpression (LPS+GDF15), and LPS with GDF15 knockdown (si-GDF15). β-actin: loading control.(HIF-1α suppression and SMAD7 induction by GDF15.) (B) Cytosolic and nuclear PKM2 protein levels. Lamin B1 (nuclear) and α-tubulin (cytosolic) markers validate fractionation efficiency. Study groups and individual replicates are identified in the figure key.(PKM2 subcellular redistribution modulated by GDF15.) (C) Immunofluorescence of PKM2 (red) and nuclei (DAPI, blue). Arrows indicate nuclear PKM2 accumulation. Scale bar: 15 μm.(Nuclear PKM2 enrichment upon LPS challenge mitigated by GDF15 and exacerbated by GDF15 knockdown.).

Article Snippet: Proteins (30 μg/lane) were separated on 10% SDS-PAGE gels, transferred to PVDF membranes (Millipore, IPVH00010), and probed with the following primary antibodies: rabbit anti-mouse UQCRC1 (Proteintech, Cat. No. 21705-1-AP, 1:1000), rabbit anti-mouse GDF15 (Proteintech, Cat. No. 27455-1-AP, 1:1000), rabbit anti-mouse HIF-1α (Proteintech, Cat. No. 20960-1-AP, 1:1000), rabbit anti-mouse SMAD7 (Proteintech, Cat. No. 725840-1-AP, 1:1000), mouse anti-mouse PKM2 (Proteintech, Cat. No. 60268-1-Ig, 1:1000), rabbit anti-mouse H3 (Proteintech, Cat. No. 17168-1-AP, 1:5000) and mouse anti-mouse β-actin (Proteintech, Cat. No. 66009-1-Ig, 1:5000).

Techniques: Expressing, Over Expression, Knockdown, Control, Fractionation, Immunofluorescence

Journal: Frontiers in Immunology

Article Title: GDF15 orchestrates mitochondrial-immune crosstalk via SMAD7-HIF-1α-PKM2 cascade to attenuate septic liver injury

doi: 10.3389/fimmu.2025.1712741

Figure Lengend Snippet: SMAD7 activation suppresses HIF-1α to mediate GDF15-dependent mitochondrial protection in LPS-challenged macrophages. (A) Pharmacological SMAD7 activation by Asiaticoside (20 μM, 48 h). β-actin: loading control. (B) HIF-1α expression under LPS challenge: LPS alone, LPS + AVV-GDF15, or LPS + SMAD7 activation (Asiaticoside). β-actin: loading control. (C) HIF-1α modulation across conditions: LPS, LPS + si-GDF15, LPS + Asiaticoside, or LPS + si-GDF15 + Asiaticoside. β-actin: loading control.

Article Snippet: Proteins (30 μg/lane) were separated on 10% SDS-PAGE gels, transferred to PVDF membranes (Millipore, IPVH00010), and probed with the following primary antibodies: rabbit anti-mouse UQCRC1 (Proteintech, Cat. No. 21705-1-AP, 1:1000), rabbit anti-mouse GDF15 (Proteintech, Cat. No. 27455-1-AP, 1:1000), rabbit anti-mouse HIF-1α (Proteintech, Cat. No. 20960-1-AP, 1:1000), rabbit anti-mouse SMAD7 (Proteintech, Cat. No. 725840-1-AP, 1:1000), mouse anti-mouse PKM2 (Proteintech, Cat. No. 60268-1-Ig, 1:1000), rabbit anti-mouse H3 (Proteintech, Cat. No. 17168-1-AP, 1:5000) and mouse anti-mouse β-actin (Proteintech, Cat. No. 66009-1-Ig, 1:5000).

Techniques: Activation Assay, Control, Expressing

Journal: Materials Today Bio

Article Title: Zwitterion nanocomposite hydrogels with bioactivity and anti-adhesion properties for rapid prevention of postoperative and recurrent adhesion

doi: 10.1016/j.mtbio.2025.101811

Figure Lengend Snippet: (a, b) Representative images of immunofluorescence staining of Smad 2/3 and Smad 7 on day 7 and day 14. (c, d) Mean fluorescence intensity (MFI) of Smad2/3 and Smad7 on day 7 and day 14, respectively. (n = 3, ∗∗∗∗p < 0.0001). (e–g) Western blot analysis and quantitative of protein expressions (Smad2/3 and Smad7) on day 7, 14. (H) Molecular mechanism of pSBLA2 hydrogel in preventing postoperative abdominal adhesions. (Created with BioRender.com ).

Article Snippet: Rabbit anti-rat Smad7 antibodies were obtained from Sanying Biotechnology.

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot

Journal: Regenerative Therapy

Article Title: Modified bFGF targeting connective tissue growth factor in the injured microenvironment improved cardiac repair after chronic myocardial ischemia

doi: 10.1016/j.reth.2025.01.006

Figure Lengend Snippet: The administration of CFBP-bFGF can alleviate MI-induced cardiac fibrosis and alleviate adverse cardiac remodeling . (A) Immunofluorescence staining and statistical analysis of collagenⅠ; (B) Immunofluorescence staining and statistical analysis of Vimentin; (C)Western blot results of TGF-β pathway and Smad7, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, n = 6.

Article Snippet: The antigen sites were blocked by 5 % skim milk powder, incubated at room temperature for 1∼2h, and incubated at 4 °C in the corresponding primary antibody overnight: Anti-FGF-2 Rabbit pAb (1:1000, BS6432, Bioworld), Anti-CTGF (1:1000, BS-0743R, Bioss), Anti-Smad7 Rabbit pAb(1:1000, 860746, zenbio), Anti-TGF beta 1 Rabbit mAb(1:1000, HUABIO, HA721143), Anti-Smad3(1:2000, Abcam, ab40854), Anti-p-Smad3 Rabbit mAb(1:1000, zenbio, R22919), Anti-β-actin Rabbit pAb (1:10,000, AP0060, Bioworld), Anti-GAPDH Rabbit pAb (1:10,000, AP0066, Bioworld).

Techniques: Immunofluorescence, Staining, Western Blot